At one time it was
thought that bacteria
and other procaryotes were essentially "bags of enzymes" with no
development of the electron microscope in the 1950s revealed the
distinct anatomical features of bacteria and confirmed the suspicion
that they lacked
a nuclear membrane. Procaryotes are cells of
simple construction, especially if compared to eucaryotes. Whereas
cells have a preponderance of organelles with separate cellular
procaryotes carry out all cellular functions as individual units.
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Secondary-structure prediction methods was continuously benchmarked, ., EVA (benchmark) . Based on these tests, the most accurate methods were Psipred , SAM,  PORTER,  PROF,  and SABLE.  The chief area for improvement appears to be the prediction of β-strands; residues confidently predicted as β-strand are likely to be so, but the methods are apt to overlook some β-strand segments (false negatives). There is likely an upper limit of ~90% prediction accuracy overall, due to the idiosyncrasies of the standard method ( DSSP ) for assigning secondary-structure classes (helix/strand/coil) to PDB structures, against which the predictions are benchmarked.